肿瘤坏死因子相关凋亡诱导配体对非小细胞肺癌细胞凋亡诱导的实验研究

Induction of apoptosis with TRAIL in non-small lung cancer

目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)对非小细胞肺癌肿瘤细胞体外诱导凋亡作用,探讨TRAIL治疗肺癌的可行性。方法 (1)单用组:单用不同浓度的TRAIL溶液处理A549并与白血病Jurkat细胞对照;(2)联合组:相同浓度的TRAIL联合不同化疗药物处理A549并与对应的单纯化疗药物对照,用MTT法及流式细胞仪检测处理前后A549细胞的凋亡发生率并比较。结果 TRAIL溶液能使部分体外非小细胞肺癌发生凋亡,TRAIL溶液浓度与细胞凋亡率在一定程度上呈正相关;联合化疗药物后的TRAIL溶液对肿瘤细胞杀灭能力显著强于单用化疗药物组,差异有统计学意义(P<0.05)。结论 TRAIL可诱导小部分非小细胞肺癌细胞体外凋亡,其凋亡作用在一定范围内与TRAIL浓度呈正相关,TRAIL联合常用化疗药物有协同促进肿瘤细胞凋亡的作用。

Objective To investigate the effect of TNF-related apoptosis inducing ligand(TRAIL) in inducing apoptosis in non-small cell lung cancer, and to explore the feasibility of TRAIL in treatment of lung cancer. Methods The single group: the cells in the two group with solutions of different concentrations of TRAIL A549 and the feasibility of TRAIL treatment between the NSCLC group and the Jurkat leukemia cell group was compared. The combination group: same concentrations of TRAIL combined with different chemotherapy treatment A549 and the corresponding chemotherapy drugs were compared, apoptosis was detected by MTT assay before and after treatment of A549 cells the incidence of parallel statistical comparison. Results TRAIL was able to make part of the non-small cell lung cancer apoptosis in vitro, TRAIL apoptosis rate and concentration had a positive correlation to some extent; TRAIL solution after combination chemotherapy drugs kill tumor cells was significantly stronger ability to eliminate drugs in the chemotherapy alone, the difference was statistically significant(P<0.05). Conclusion TRAIL can induce lung cancer cells in vitro,small portion of non-small cell apoptosis of apoptosis within a certain range of concentrations are positively related with TRAIL, TRAIL combined chemotherapy drugs have synergistic apoptosis of tumor cells.