摘要
目的:探讨喉癌患者组织样本中蛋白磷酸酶2A及其相关蛋白在癌组织与癌旁组织之间的表达差异,建立可用于临床进行喉癌患者预后分层的多指标预测体系。创新点:以蛋白磷酸酶2A为核心,探讨系列肿瘤转移相关指标参与喉癌发生发展的分子机制。方法:收集2012~2015年间浙江大学医学院附属第二医院耳鼻咽喉科28例喉鳞状细胞癌患者的临床组织样本,提取总蛋白后进行免疫印迹分析。结论:与癌旁正常组织相比,喉癌组织中phosphoPP2A/C(Y307)、α4、CIP2A、Akt、ezrin、phosphoezrin(T567)、14-3-3以及FAK蛋白水平均显著升高,而phospho-Akt(T308)蛋白水平则显著降低。
Laryngeal squamous cell carcinoma(LSCC)is the most common type of head and neck squamous cell carcinoma(HNSCC)worldwide.Protein phosphatase 2A(PP2A)dysfunction has been widely reported in a broad range of malignancies due to its distinctive role in miscellaneous cellular processes.However,it is poorly understood whether aberrant alterations of PP2A are involved in the network of oncogenic events in LSCC.Here,we detected a panel of PP2A-associated proteins using western blot in both laryngeal squamous cell carcinoma tissues and paired adjacent normal tissues from patients(Data S1).We found that phospho-PP2A/C(Y307),α4,cancerous inhibitor of protein phosphatase 2A(CIP2A),Akt,ezrin,phospho-ezrin(T567),14-3-3,and focal adhesion kinase(FAK)showed increased expression levels in carcinoma tissues relative to normal tissues,while phospho-Akt(T308)showed decreased levels.Our study,thus,provides a rationale for targeting PP2A to develop novel therapies and proposes a combination of interrelated biomarkers for the diagnostic evaluation and prognosis prediction in LSCC.
基金
the Medical Scientific Research of Zhejiang Province(Nos.2009A044 and 2015KYA055)
the Natural Science Foundation of Zhejiang Province(No.LY13H130001)
the Program of Science Technology Department(No.2013C33208)
the Program of Traditional Chinese Medicine Administration of Zhejiang Province(No.2012ZA085)
China
