免疫亲合色谱及在农药残留分析中的应用

Immunoaffinity Chromatography and its Application in Pesticide Residue Analysis

免疫亲合色谱可用于复杂样本中微量目标分析物的选择性分离富集。综述了免疫亲合色谱的基本原理、方法的建立过程(包括抗体的制备、固相基质的筛选、抗体与基质的偶联、免疫亲合色谱条件的优化)、免疫亲合色谱在农药残留分析中的应用现状及发展趋势。

Immunoaffinity chromatography (IAC) could be used for the separation and enrichment of trace target small molecular compounds such as pesticides in complicated samples with high specificity. The review is on the principle of IAC, the developmental procedure of IAC(including the preparation of antibody, the (selection) of matrix, the coupling of antibody to matrix, and the optimization of IAC condition), the (application) of IAC in pesticide residue analysis and the trend of this technology.