河底沉积物培养耐酸产甲烷颗粒污泥的试验研究

Studies on cultivation of acid-tolerant methanogenic granular sludge using river sediments.

利用河底沉积物作为接种污泥,在一个3.1L的EGSB反应器中进行培养耐酸产甲烷颗粒污泥的试验研究.结果表明,EGSB反应器在pH6.0,出水碱度低于400mg CaCO3/L,容积负荷5.3kg COD/(m3d)的条件下培养出具有良好沉降性能和产甲烷活性的耐酸颗粒污泥.形成耐酸颗粒污泥后,EGSB反应器在pH5.8～6.0,进水COD 3000mg/L,容积负荷5.2kg COD/(m3d)的条件下稳定运行29d,COD去除率平均为89.2%,出水总碱度仅为264.4mg CaCO3/L,沼气中甲烷的含量约为56 9%.扫描电镜观察发现颗粒污泥内部存在成簇生长的索氏甲烷丝菌.

Studies on the cultivation of acid-tolerant methanogenic granular sludge in a 3.1L expanded granular sludge bed (EGSB) reactor using river sediments as seed sludge was conducted. In the reactor, acid-tolerant granular sludge with good settling ability and methanogenic activity was cultivated under the conditions of pH 6.0, effluent alkalinity lower than 400mg CaCO3/L, loading rate 5.3kg COD/(m3d). Thereafter, under the condition of pH 5.8~6.0, influent COD3000mg/L and loading rate 5.2kg COD/(m3d), the reactor operated stably for 29 days with the average COD removal rateof 89.2%, effluent total alkalinity of 264.4mg CaCO3/L and the methane content of about 56.9% in biogas. Investigated under scanning electronic microscope, many Methanothrix soehngenii were found aggregated in cluster in the inner areas of granular sludge.