GSK-3β和MIF蛋白在下咽癌组织中的表达及意义

Expression and significance of GSK-3β and MIF in hypopharyngeal carcinoma

目的研究糖原合成酶激酶-3β(giycogen syntlase kinase-3β,GSK-3β)和巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)蛋白在下咽癌组织中的表达,探讨二者在下咽癌发生、发展中的作用及二者相关性。方法应用免疫组织化学方法检测46例下咽癌组织、癌旁组织及20例下咽正常黏膜组织中GSK-3β和MIF蛋白表达情况。结果①GSK-3β蛋白在下咽癌组织中的阳性表达率明显高于癌旁组织和正常下咽黏膜组织(P<0.05);②MIF蛋白在下咽癌组织中的阳性表达率明显高于癌旁组织和正常下咽黏膜组织(P<0.05);③GSK-3β蛋白在下咽癌组织中的表达与淋巴结转移、临床分期、病理分级有相关性(P<0.05);④MIF蛋白在下咽癌组织中的表达与淋巴结转移、临床分期有相关性(P<0.05);⑤GSK-3β和MIF蛋白在下咽癌组织中表达无相关性。结论GSK-3β和MIF蛋白表达与下咽癌的发生、发展可能有关。

Objective To investigate the expressions of glycogen syntlase kinase-3β(GSK-3β)and macrophage migration inhibitory factor(MIF)in hypopharyngeal carcinoma and to discuss their relevance and the roles in carcinogenesis and development of this tumor.Methods Immunohistochemical technique was used to detect the protein expressions of GSK-3βand MIF in specimens of 46 hypopharyngeal carcinoma tissues and corresponding para-carcinoma tissues as well as 20 normal hypopharyngeal mucosae.Results①The expression of GSK-3βprotein in hypopharyngeal carcinoma tissues was significantly higher than those in the para-carcinoma tissues and normal mucosae(both P<0.05).②The expression of MIF protein in hypopharyngeal carcinoma tissues was also significantly higher than those in the para-carcinoma tissues and normal mucosae(both P<0.05).③The expression of GSK-3βprotein in hypopharyngeal carcinoma was correlated with lymph node metastasis,clinical stage and histological grade of the tumor respectively(all P<0.05).④The expression of MIF protein in hypopharyngeal carcinoma was correlated with lymph node metastasis and clinical stage of the tumor(both P<0.05).⑤There was no correlation between the expressions of GSK-3βand MIF in hypopharyngeal carcinoma.Conclusion The expressions of GSK-3βand MIF may contribute to the carcinogenesis and development of hypopharyngeal carcinoma.