逆转录病毒介导p53基因对食管癌细胞株的生长抑制作用

The growth-suppressive effect of retrovirus-mediated wild-type p53 gene transfer on human esophageal cancer cell line

目的 观察人类野生型p5 3(wtp5 3)基因对人食管癌细胞系的抑制作用。方法 用逆转录病毒为载体将外源性wtp5 3基因导入人食管癌细胞系ECA10 9,通过体外及小鼠体内实验研究转入基因的表达及对肿瘤的抑制作用。结果 p5 3在转染细胞ECA10 9/p5 3中表达水平提高。外源性wtp5 3基因的导入和表达能使ECA10 9细胞的生长速率减低 ,软琼脂集落形成能力下降 ,G0 +G1期细胞比例增加、S期细胞比例降低 ,凋亡指数升高 ,裸鼠体内成瘤能力明显下降。结论 逆转录病毒载体介导的外源性野生型p5 3能够抑制人食管癌细胞生长。

Objective To observe the growth-suppressive effect of human wild-type p53(wtp53) gene on human esophageal cancer cell line. Methods The retroviral vector to introduce exogenous wtp53 gene into human esophageal cancer cell line ECA109, the gene expression and tumor inhibition were studied in vitro and in vivo. Results The expression of p53 in transfected cell lines (ECA109/p53) was increased. The introduction and expression of exogenous wtp53 gene resulted in that the growth rate and the ability to form colony in soft agar were greatly inhibited in ECA109/p53 cells versus ECA109/neo and ECA109 cells. The G0+G1 ratio increased and S ratio decreased in cell cycle distribution, and apoptosis index significantly rose in the ECA109/p53 cells, which were confirmed by FCM analyzing. The tumorigenicity of ECA109/p53 cells in nude mice was obviously suppressed by exogenous p53. Conclusion Exogenous wtp53 mediated by retroviral vector could inhibit the growth of human esophageal cancer cell.