HPLC-ECD法对精神分裂症患者脑脊液单胺类神经递质的检测

HPLC-ECD method for the determination of CSF level of monoamine neurotransmitter in patients with schizophrenia

目的 探讨精神分裂症患者脑脊液 (CSF)中多巴胺 (DA)、高香草酸 (HVA)含量的变化及立体定向手术对其的影响。方法 立体定向手术治疗 2 2例精神分裂症患者 ,高效液相色谱 电化学HPLC ECD法检测CSF中的DA及HVA水平。结果 精神分裂症患者手术前CSF中DA[(3.2 3± 0 .36 ) μmol/L]、HVA[(1.99± 0 .4 9)μmol/L]水平显著高于对照组 [(2 .4 4± 0 .32 ) μmol/L、(1.4 1± 0 .37) μmol/L](P <0 .0 1) ;手术后DA[(2 .4 9± 0 .35 ) μmol/L]、HVA[(1.4 2± 0 .2 8) μmol/L]水平明显下降 (P <0 .0 1)。 结论 支持精神分裂症中枢DA亢进假说 ,立体定向手术具有明显的DA传导阻滞作用。

Objective To study the effect of stereotactic operation on CSF levels of dopamine(DA), homovanillic acid(HVA) in patients with schizophrenia. Methods 22 patients with schizophrenia were treated with stereotactic operation. CSF levels of DA and HVA were detected with high performance liquid chromatography and electrochemical detector(HPLC ECD). Results CSF levels of DA[(3.23±0.36) μmol/L] and HVA[(1.99±0.49) μmol/L] were significantly higher( P <0.01) in patients with schizophrenia before operation than that of nomal control [(2.44±0.32 μmol/L), (1.41±0.37) μmol/L]( P <0.01). DA[(2.49±0.35) μmol/L] and HVA[(1.42±0.28) μmol/L] were significantly lower( P <0.01) after operation than before. Conclusions The result supports the hypothesis of DA excessive in patients with schizophrenia. Stereotactic operation has evident DA nerve block action.