氧化型低密度脂蛋白IgG型免疫复合物的检测及临床应用

Detection and clinical significance of oxidatively modified low-density-lipoprotein circulating immune complex

目的 探讨血清氧化修饰型低密度脂蛋白免疫复合物 (OX LDL IC)的检测方法及其在动脉粥样硬化发病机制中的作用。方法 取纯化单克隆抗OX LDL抗体包被反应板以识别循环免疫复合物中的抗原部分 ,以羊抗人IgG酶结合物识别循环免疫复合物中的抗体部分 ,采用ELISA检测 90例不同类型冠心病患者血清中OX LDL IC水平 ,并进行统计学分析。结果 冠心病各组患者血清OX LDL IC水平均明显高于对照组 ,并表现出随病情加重而逐渐增加的趋势。结论 OX LDL IC的形成增强了OX LDL致动脉粥样硬化的能力 。

Objective To establish a method to detect oxidatively modified low density lipoprotein circulating immune complex (OX LDL IC) and explore its pathogenic role on the development of atheriosclerosis. Methods A bi site ELISA method to detect IgG bound OX LDL IC was established. The antigen portion of OX LDL IC was captured by a purified monoclonal antibody to OX LDL, and antibody portion was recognized by enzyme conjugated anti human IgG(goat). 90 patients with CHD were involved in this study and the levels of OX LDL IC were detected. Results In spite of a large dispersion in the quantity of OX LDL IC, the levels in patients were significantly higher than that in controls, and the more severe atherosclerosis, the higher the levels. Conclusions Oxidative modification of LDL and the formation of OX LDL IC could increase its atherogenicity, and were associated with the presence or progression of atherosclerosis.