卵巢癌患者血清MMP12、MMP13水平与临床病理参数和预后的关系

Levels of serum MMP12 and MMP13 in patients with ovarian cancer and the relationships with clinicopathological parameters and prognosis

目的探讨上皮性卵巢癌患者血清基质金属蛋白酶12 (MMP12)、基质金属蛋白酶13 (MMP13)表达水平与上皮性卵巢癌临床病理参数和预后关系。方法选取2011年10月-2013年10月收治的70例上皮性卵巢癌患者作为上皮性卵巢癌组,65例卵巢良性疾病患者作为卵巢良性疾病组,62例健康体检女性作为健康对照组。采用酶联免疫吸附试验(ELISA)双抗体夹心法检测血清MMP12、MMP13水平,并分析其与上皮性卵巢癌患者临床病理参数及5年总生存率(OS)关系。结果上皮性卵巢癌组患者血清MMP12、MMP13水平均明显高于卵巢良性疾病组患者和健康对照组女性,差异有统计学意义(P<0. 05);卵巢良性疾病组患者高于健康对照组女性,但差异无统计学意义(P> 0. 05)。血清MMP12、MMP13表达水平与上皮性卵巢癌患者组织分型、分化程度、FIGO分期、腹腔积液量、淋巴结转移有关(P<0. 05)。上皮性卵巢癌患者血清MMP12、MMP13低表达者5年OS均显著高于高表达者,差异有统计学意义(P<0. 05);FIGO分期、腹腔积液量、MMP12表达、MMP13表达均是影响上皮性卵巢癌患者预后的独立危险因素。结论上皮性卵巢癌患者血清MMP12、MMP13均高表达,二者表达与FIGO分期、腹腔积液量等临床病理参数及预后有关,可作为判断预后的指标。

Objective To explore the expression levels of serum matrix metalloproteinase 12(MMP12)and matrix metalloproteinase13(MMP13)in patients with epithelial ovarian cancer,analyze the relationships between MMP12,MMP13 and clinicopathological parameters and prognosis of epithelial ovarian cancer.Methods From October 2011 to October 2013,seventy patients with epithelial ovarian cancer were selected as epithelial ovarian cancer group,sixty-five patients with benign ovarian diseases were selected as benign ovarian disease group,and sixty-two women who underwent healthy physical examination during the same period were selected as healthy control group.Enzyme-linked immunosorbent assay(ELISA)double antibody sandwich method was used to detect the levels of serum MMP12 and MMP13,the relationships between MMP12,MMP13 and clinicopathological parameters,five-year survival rate of patients with epithelial ovarian cancer were analyzed.Results The levels of serum MMP12 and MMP13 in epithelial ovarian cancer group were statistically significantly higher than those in benign ovarian disease group and healthy control group(P<0.05).The levels of serum MMP12 and MMP13 in benign ovarian disease group were higher than those in healthy control group,but there was no statistically significant difference(P>0.05).The expression levels of serum MMP12 and MMP13 were correlated with histological typing,differentiation degree,FIGO stage,peritoneal effusion volume,and lymph node metastasis in patients with epithelial ovarian cancer(P<0.05).In epithelial ovarian cancer group,five-year survival rate of patients with low expression levels of serum MMP12 and MMP13 was statistically significantly higher than that of patients with high expression levels of serum MMP12 and MMP13(P<0.05).FIGO staging,peritoneal effusion volume,MMP12 expression,and MMP13 expression were independent risk factors affecting the prognosis of patients with epithelial ovarian cancer.Conclusion The levels of serum MMP12 and MMP13 are high in patients with epithelial ovarian cancer,the expressions are correlated with clinicopathological parameters(such as FIGO staging,peritoneal effusion volume)and prognosis of patients with epithelial ovarian cancer,which can be used to predict the prognosis.