骨髓间充质干细胞成骨分化的研究与进展

Research and development of osteogenic differentiation of bone marrow mesenchymal stem cells

超临界大面积或大段骨缺损仍是临床面临的难题,研究者们致力于研发人工骨材料,但为了解决人工骨材料成骨效应不良的问题,人们越来越关注骨髓间充质干细胞在骨组织工程中的应用。本综述以骨髓间充质干细胞、成骨分化、成骨细胞、临床应用"Bone marrow mesenchymal stem cells,osteogenesis differentiation,osteoblasts cell,clinical application"为检索词,应用计算机搜索CNKI数据库、万方数据库、维普数据库、PUBMED数据库,全面归纳总结骨髓间充质干细胞的分离培养、骨髓间充质干细胞鉴定方法、成骨诱导方法、成骨分化鉴定及其在临床中的应用,为证实其作为种子细胞治疗骨组织疾病提供理论依据。学者们初步研究了骨髓间充质干细胞结合移植、组织工程等技术来治疗骨和软骨缺损、骨关节炎、股骨头坏死等疾病,均取得了较好的临床疗效。但是骨髓间充质干细胞会有一定的优缺点,其临床试验及远期疗效的验证还需进一步深入研究。

Supercritical large area or large segmental bone defects are still a clinical problem. Researchers are committed to the development of artificial bone materials,but in order to solve the problem of poor bone formation of artificial bone materials,people are paying more and more attention to application of bone marrow mesenchymal stem cells in bone tissue engineering. In this review,bone marrow mesenchymal stem cells,osteogenic differentiation,osteoblasts cells,clinical application were used as keywords to search CNKI database,Wanfang database,Weipu database and PUBMED database by computer. The isolation and culture of bone marrow mesenchymal stem cells,bone marrow mesenchymal stem cell identification method,osteogenic induction method,osteogenic differentiation identification and clinical applicationt were comprehensively summarized in order to provide a theoretical basis for its use as a seed cell in the treatment of bone tissue diseases. Scholars have preliminarily studied the treatment of bone and cartilage defects,osteoarthritis,femoral head necrosis and other diseases with bone marrow mesenchymal stem cells combined with transplantation,and obtained good clinical efficacy.However,bone marrow mesenchymal stem cells have certain advantages and disadvantages,and further clinical studies and long-term efficacy verification are needed.