自噬相关蛋白及基因在非小细胞肺癌中的表达及其意义

Expression of autophagy-related proteins and genes in patients with non-small cell lung cancer

目的检测非小细胞肺癌患者肺组织标本中自噬相关基因和蛋白的表达情况。方法标本取自临床疑诊为非小细胞肺癌行手术切除的肺组织,其中肺癌组为病理诊断为非小细胞肺癌的组织,正常组为远离肺部病灶5 cm以外的组织(肺癌组29例,正常组32例)。采用Western blot检测自噬相关蛋白ATG5、LC3B、p62的蛋白表达情况;采用实时–聚合酶链反应(RT-PCR)方法检测肺癌组织、正常组织中自噬相关基因ATG5、p62mRNA的表达情况。结果 Western blot结果显示自噬相关蛋白ATG5和p62在肺癌组中表达量增加,显著高于正常组(P<0.05);LC3B在肺癌组织中表达量显著低于正常组织(P<0.05)。RT-PCR结果显示肺癌组的ATG5、p62 mRNA水平均显著高于正常组(P<0.05)。ATG5、LC3B、p62的蛋白相对表达量在性别、年龄、吸烟史、肿瘤部位、肿瘤大小、肿瘤病理类型、分化程度以及肿瘤TNM分期各组间差异无统计学意义(P>0.05)。ATG5的蛋白表达量在淋巴结是否转移组间差异有统计学意义(P<0.05);LC3B、p62蛋白表达量在淋巴结是否转移组间差异无统计学意义(P>0.05)。结论自噬在肺癌的发生中发挥一定的作用。自噬相关基因和蛋白若能够正确有效调控,可能会对未来临床治疗方面提供新的依据和靶点。

Objective To investigate the expression of autophagy-related genes and proteins in the lung tissues of patients with non-small cell lung cancer(NSCLC).Methods Pulmonary tissues were obtained from the surgically resected lung tissues of patients with NSCLC who were clinical diagnosed.The lung cancer tissues were derived from the pathologically diagnosed NSCLC and the normal tissues were from lung tissues 5 cm away from the lung lesions(29 cases in the lung cancer group and 32 cases in the normal group).The expression of autophagy-related proteins ATG5,LC3 B,and p62 in lung tissues were measured by Western blot,and mRNA expression of ATG5 and p62 in the lung tissues were measured by real-time PCR.Results Western blot analysis showed that the expression of ATG5 and p62 in lung cancer group were significantly higher than those in normal group(P<0.05).However,the expression of LC3 B in lung cancer group was significantly lower than that in normal group(P<0.05).Real-time PCR analysis found that the mRNA expression of ATG5 and p62 in lung cancer group were significantly higher than those in normal group(P<0.05).The expression of ATG5,LC3 B and p62 had no relationship with gender,age,smoking history,tumor location,tumor size,clinicopathological classification,differentiation or TNM stage.The expression of ATG5 had statistical significance in lymph node metastasis(P<0.05),but there was no difference for LC3 B or p62 in lymph node metastasis(P>0.05).Conclusions Autophagy plays a role in the tumorigenesis of lung cancer.If it’s possible to regulate and control autophagy-related genes and proteins effectively,it may supply new insights or targets into treatment for lung cancer patients.