不同异嗜性粒细胞与淋巴细胞比值雏鸡对鼠伤寒沙门菌免疫应答反应差异

The Difference in Immune Respone of Chicks with Different Heterophil to Lymphocyte Ratio Against Salmonella Typhimurium

研究旨在观察不同异嗜性粒细胞与淋巴细胞比值(Heterophil/lymphocyte,H/L)雏鸡对鼠伤寒沙门菌免疫应答反应的差异,挖掘最佳抗病能力的H/L值。京星黄鸡根据表型选择8个世代,每个世代的公母比例为1:3,后代按H/L比值分为高H/L组、中H/L组和低H/L组,于7日龄口服鼠伤寒沙门菌,感染后3、7、12、26 d进行死亡率、H/L值、组织载菌量和血清介质指标的检测。结果显示:感染沙门菌后,低H/L组的死亡率(10.00%)低于中H/L组(13.30%)和高H/L组(31.11%);低H/L组肝脏组织载菌量在3、7 d显著低于高H/L组(P<0.05),12 d时差异不显著(P>0.05)。低H/L组血清白细胞介素6(IL-6)的浓度在3和7 d显著高于高H/L组(P<0.05),低H/L组血清肿瘤坏死因子-α(TNF-α)和白三烯B4(LTB-4)的浓度在3 d显著高于高H/L组(P<0.05)。表明具有低H/L值的雏鸡对鼠伤寒沙门菌感染的免疫应答反应更强,有更好的沙门菌抗性。

The purpose of this study was to observe the differences in immune response of chicks with different Heterophil/lymphocyte(H/L) ratio against Salmonella Typhimurium(ST), and to find out the best disease resistance.Jingxinghuang H line has been chosen for 8 generations. The ratio of male to female in each generation is 1:3. The descendants were divided into high H/L group, medium H/L group and low H/L group according to the ratio. The birds were inoculated ST at 7 days of age, mortality at 3, 7, 12, 26 days after infection, H/L ratio, bacteria burden of tissue and serum media indicators were detected. The results showed that after ST infection, the mortality rate in low H/L group(10.00%) was lower than that in middle H/L group(13.30%) and high H/L group(31.11%)(P<0.05);The counting of ST in liver in low H/L group was significantly lower than that in high H/L group at 3 and 7 days after infection(P<0.05),and the difference was not significant at 12 days after infection(P>0.05). The serum levels of interleukin-6(IL-6) in low H/L group were significantly higher than those in high H/L group at 3 and 7 days after infection(P<0.05), and the concentrations of tumor necrosis factor-α(TNF-α) and leukotriene B4(LTB-4) were significantly higher than those in high H/L group at 3 days after infection(P<0.05). It′ s suggested that chicks with low H/L ratio had a stronger immune response to ST infection and better Salmonella resistance.