摘要
为研究武定鸡和科宝肉鸡成骨细胞分化、矿化及肿瘤坏死因子α(TNF-α)基因表达差异,试验通过提取武定鸡和科宝肉鸡骨间充质干细胞,并诱导分化为成骨细胞,检测成骨细胞分化、矿化差异及TNF-α基因表达差异。结果显示:与科宝肉鸡相比,在骨间充质干细胞诱导为成骨细胞过程中,武定鸡成骨细胞矿化能力较强,且TNF-α基因表达量显著低于科宝肉鸡。研究表明:TNF-α可通过调控成骨细胞分化来影响矿化过程,进而影响骨骼强度。可见,TNF-α基因对家鸡成骨细胞矿化起着重要的作用。
In order to study differences on differentiation,mineralization and TNF-αgene expression of osteoblast in Wuding chicken and Cobb broiler,bone mesenchymal stem cells from Wuding chicken and Cobb broiler were obtained to induce into osteoblasts.Differentiation and mineralization of osteoblast and expression of TNF-αin osteoblasts were detected.The results showed that compared with the Cobb broiler,in the process of induced into osteoblasts,the mineralization ability of Wuding chicken was stronger than that in Cobb broiler.And the expression of TNF-αgene in osteoblasts from Wuding chicken was significantly lower than that in Cobb broiler.It indicated that TNF-αgene could affect bone strength by regulating osteoblast differentiation to influence mineralization process.So,it can be seen that TNF-αgene plays an important role in the mineralization of chicken osteoblasts.
作者
豆腾飞
赵净颖
王秋婷
段小花
李涛
徐志强
谷大海
葛长荣
贾俊静
DOU Tengfei;ZHAO Jingying;WANG Qiuting;DUAN Xiaohua;LI Tao;XU Zhiqiang;GU Dahai;GE Changrong;JIA Junjing(Faculty of Animal Science and Technology,Yunnan Provincial Key Laboratory of Animal Nutrition and Feed,Yunnan Agricultural University,Kunming,Yunnan 650201;Yunnan University of TCM,Kunming,Yunnan 650201;College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201)
出处
《中国家禽》
北大核心
2019年第6期7-10,共4页
China Poultry
基金
国家自然科学基金项目(31572359
U1702232
31560618)
云南省自然科学基金重点项目(2016FA046)
云南省科技厅南希.莱恩院士工作站(2017IC048)
云岭学者和云岭产业技术领军人才项目
云南农业大学研究生科技创新项目(2016ykc8)
