小儿消化性溃疡患者外周血单个核细胞中转录因子ROR γ tmRNA的表达

Expression on transcription factor of RORγt m RNA in peripheral blood mononuclear cells among children with peptic ulcer

目的探讨Th17细胞及其转录因子RORγt m RNA在小儿消化性溃疡致病机制中的作用。方法采用实时荧光定量PCR法检测28例小儿消化性溃疡患者和28例正常人对照组外周血单个核细胞中RORγt m RNA的表达水平。结果小儿消化性溃疡患者和正常人对照RORγt m RNA两组比较差异有高度统计学意义(t=-5.772,t=-6.105,P<0.001)。结论转录因子RORγt高表达可能在小儿消化性溃疡致病机制中具有重要作用。

Objective To explore the role of Th17 cell and its transcription factor of RORγt m RNA in the pathogenic mechanism of peptic ulcer in children. Methods Real-time fluorescence quantification PCR was adopted to detect the expression levels of RORγt m RNA in peripheral blood mononuclear cells among 28 children with peptic ulcer and 28 healthy controls. Results The expression level differences of RORγt m RNA in sufferers with peptic ulcer and the healthy controls were of high statistical significance(t =-5.772,t =-6.105,P <0.001).Conclusion The high expression on transcription factor of RORγt m RNA may play an important role in pathogenic mechanism of peptic ulcer in children.