三个苎麻脱胶高效菌种的生长条件优化

Optimization of the Culture Conditions for Three Efficient Strains Used in Ramie Bio-degumming

研究3个苎麻脱胶高效菌种的最佳生长培养条件。利用分光光度计,以菌液浊度OD600为指标,对苎麻脱胶高效菌种活化条件的主要参数(起始p H、接种量、培养温度和培养时间)进行研究。通过单因素和正交试验,获得3个苎麻脱胶高效菌种活化条件的最优组合。结果表明:Hn1-1菌种的最佳生长条件为起始p H 6.0、接种量5%、培养温度37℃、培养时间8 h;Hn2-2菌种的最佳生长条件为起始p H 8.0、接种量6%、培养温度30℃、培养时间7 h;Hn6-2菌种的最佳生长条件为起始p H 6.0、接种量7%、培养温度35℃、培养时间6 h。通过优化培养条件,3个菌种的种子液生物量提高到1.5~2.0倍,为其在苎麻脱胶的进一步应用提供科学依据。

The optimal culture conditions of three efficient strains used in ramie bio -degumming were researched. Cell suspension turbidity (optical density at 600 nm,OD600 )was measured using spec-trophotometer to investigate the optimal initial pH,inoculation amount,culture temperature and time of the activation conditions for efficient strain used in ramie bio-degumming. Using one-factor-at-a-time combined with orthogonal array design method,optimal combination of the activation conditions for the three aimed efficient strains were found. Results showed that Hn1 -1 strain achieved the highest bio-mass with inoculation amount 5% in 8 h of incubation at initial pH 6.0 and 35.5℃,Hn2 -2 strain a-chieved the highest biomass with inoculation amount 6%in 7 h of incubation at initial pH 8.0 and 30℃, and Hn6-2 strain achieved the highest biomass with inoculation amount 7%in 6 h of incubation at ini-tial pH 6.0 and 35℃in the given conditions. Therefore,it can provide scientific basis for further appli-cation in ramie degumming as for the three improved liquid biomass (as 1.5 ~2.0 folds as that of original OD600 )by optimizing the culture conditions.