摘要
为获得抗晚疫病的转基因马铃薯植株,采用根癌农杆菌介导法,以微型薯薯片为外植体,将萝卜抗菌肽基因(AFP)导入马铃薯栽培品种夏波蒂(Shepody)中,通过草甘膦抗性筛选和PCR检测获得了4株转基因试管苗,并经黑暗诱导获得了微型薯。采用离体叶片接种法,对微型薯长出的叶片进行抗晚疫病鉴定,结果表明这4个株系的抗病性均有一定程度的提高。
The aim of the study is to obtain the transgenic potato(Solanum tuberosum L.)lines conferring resistance to late blight.AFP(antifungal protein)gene was introduced into potato susceptible cultivar'Shepody'via Agrobacterium tumefaciens-mediated transformation,using micro-tuber discs as explants.Four positive plantlets were obtained by PCR assay and the glyphosate selection.The micro-tubers were introduced in the dark and then planted in the field to grow into complete plants for the identification of disease resistance.Using detached leaf assay,4 transgenic plant lines were identified for late blight resistance.The result showed that their disease resistance was higher than the non-transgenic ones.
出处
《中国蔬菜》
北大核心
2012年第03X期68-73,109,共7页
China Vegetables
基金
甘肃省科技厅项目(1011NKCA079)
