二倍体马铃薯花药培养再生体系的优化

Optimization of Regeneration System for Diploid Potato Anther Culture

选取与花药培养相关的因素及水平进行探讨,以优化二倍体马铃薯花药培养的再生体系。结果表明:二倍体马铃薯花药培养的最适基因型为BD40-2、BD54-8和BD10-7;对于促进花药诱导,低温、热激、离心、甘露醇4种预处理之间存在极显著差异,确定最佳的预处理为低温6 d、热激4 d、离心4 000 r.min-1、甘露醇处理2 d;2,4-D为1.0 mg.L-1、6-BA为0.1 mg.L-1的激素配比的花药诱导率最高;碳源选用麦芽糖,浓度为3%;培养基中添加1 mmol.L-1STS诱导的愈伤花药数显著高于培养基中添加50mg.L-1AgNO3。

In this experiment,factors and levels related to anther culture were selected to discuss the optimization of regeneration system for diploid potato(Solanum tuberosum L.)anther culture.The results indicated that the optimal genotypes for diploid anther culture were BD40-2,BD54-8 and BD10-7.Four pretreatments including low temperature,heat shock,decentralization and mannitol had significant differences on callus inducing.And the optimal combination were low temperature for 6 days,heat shock for 4 days,centrifugation for 4 000 r.min-1and mannitol for 2 days.The horhome combination was 2,4-D 1.0 mg.L-1 + 6-BA 0.1 mg.L-1,which has the highest rate of anther induction.Conformable carbon source was barley sugar at the level of 3%.Adding 1 mmol.L-1 STS in medium had advantages in callus inducing over 50 mg.L-1AgNO3.