摘要
山东省济南地区2011年嫁接西瓜严重发生倒瓤,通过实地调查,初步判断为病毒侵染引起,经对采集的80份西瓜病样进行ELISA(Enzyme-Linked Immuno-Sorbnent Assay)和RT-PCR(Reverse Transcription Polymerase Chain Reaction)验证。结果表明:经ELISA检测80份西瓜样本中有70份感染了黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaicvirus,CGMMV),证明山东省济南地区嫁接西瓜严重发生倒瓤是由CGMMV引起的。
The blood flesh of watermelon〔Citrullus lanatus(Thunb.)Matsum. et Nakai〕out broke on grafted watermelon in Jinan area,Shandong Province in 2011.The viral pathogen was suspected as its cause after survey in fields.Eighty samples of grafted watermelon leaves and fruits with viral symptoms and non-symptom collected from Jinan area,Shandong Province were detected by ELISA and RT-PCR.The results showed that 70 of these 80 samples were infected by Cucumber green mottle mosaic virus,preliminary confirming that CGMMV was the cause of blood flesh of watermelon in Jinan area,Shandong Province.
出处
《中国蔬菜》
北大核心
2012年第05X期75-79,共5页
China Vegetables
基金
国家现代农业产业技术体系建设专项(CARS-26-13)
