摘要
对北京市昌平区采集的35个草莓根腐病病样进行病原鉴定,结果分离获得13个菌株,根据培养特征和形态学鉴定,确定分离到的菌株均为尖孢镰刀菌(Fusarium oxysporum);选取真菌通用引物ITS1/ITS4扩增菌株的rDNA-ITS序列并进行测序,通过与GenBank数据库序列比对,分子鉴定结果与形态学一致。将分离到的菌株回接到健康草莓幼苗上,植株表现出与田间发病相似的症状,重新分离能够得到相同的病原物。基于形态学、分子生物学鉴定及致病性检测,最终确定北京市昌平区草莓根腐病的病原菌为Fusarium oxysporum。
A high incidence of strawberry root rot was observed in Changping District of Beijing in April 2011 and 35 samples of affected plants were collected at random.Thirteen strains were taken at the colony edge for subculture and purification.The purified isolates were primarily identified based on morphological and colony characteristics.It was found that all strains isolates were similar in morphology and colony.Analysis of rDNA-ITS sequence of isolates indicated that the strains were Fusarium oxysporum.The healthy strawberries were used for fungal pathogenicity test.All isolates had pathogenicity.Symptoms became to appear 2 weeks after inoculation.The pathogens were recovered from strawberry roots,confirming Koch's postulates.Based on the morphological characteristics,pathogenicity test and molecular methods,strawberry root rot pathogens were identified as Fusarium oxysporum.
出处
《中国蔬菜》
北大核心
2012年第06X期52-56,共5页
China Vegetables
基金
北京市农业局科技项目
