摘要
为了获得与大白菜抗根肿病基因紧密连锁的分子标记,以高抗大白菜根肿病的F1金锦2号(编号A00645)及其自交F2群体197个单株为材料,通过根肿病接种鉴定和遗传分析,发现该材料中根肿病抗性由显性单基因控制。利用分离群体分组分析法(BSA)和InDel分子标记技术,在F2分离群体中构建抗感病池,筛选了720对InDel引物,多态性标记进一步单株验证并利用JoinMap4.0软件统计分析,结果获得与大白菜抗根肿病基因连锁的InDel标记9个,其中最近的两侧标记为BrID90269和BrID11683,遗传距离分别为2.0cM和2.5cM。该抗病基因定位在大白菜染色体A8的Scaffold10上。
In order to obtain molecular markers closely linked to the clubroot resistance gene in Chinese cabbage〔Brassica campestris L.ssp.pekinensis(Lour)Olsson〕,a segregating F2 population with 197 individuals was constructed by selfing of a F1 hybrid A00645,which is highly resistant to clubroot.Clubroot resistance tests and genetic analysis indicated that the inheritance of clubroot resistance in this material was controlled by a single dominant gene.Based on bulked segregating analysis(BSA)and InDel technique,a total of 720 pairs of InDel primers were screened between the resistant and susceptible bulks constructed in F2 population.After further tests among F2 individuals and linkage analysis by JoinMap4.0,9 InDel markers linked to clubroot resistance gene were obtained.The flanking markers BrID90269 and BrID11683 closely linked to the resistance gene were located on each side,at genetic distances of 2.0 cM and 2.5 cM,respectively.This clubroot resistance gene was mapped on Scaffold10,which is in linkage group A8 of Chinese cabbage.
出处
《中国蔬菜》
北大核心
2012年第07X期31-35,共5页
China Vegetables
基金
国家大宗蔬菜产业技术体系(CARS-25-A-01)
主要蔬菜作物分子育种与功能基因组研究项目(2012AA100100)
农业部园艺作物遗传改良重点开放实验室项目
