菊芋基因组DNA提取方法的比较

Comparison of Genomic DNA Extraction from Helianthus tuberosus L

采用改良CTAB法、改进的高盐SDS法及两种植物基因组DNA提取试剂盒法等4种方法提取菊芋基因组DNA,并进行电泳分析、质量检测及ISSR引物扩增检测。结果表明,改良CTAB法优于其他3种方法,提取的DNA质量较好、纯度较高,能够满足ISSR-PCR扩增要求。

The genomic DNA was isolated from the leaf of Helianthus tuberosus L.by the improved CTAB method,improved high salt SDS method and 2 genomic DNA kits.The DNA quality was examined by agarose gel electrophoresis and PCR amplification of ISSR.The result showed that the improved CTAB method was obviously better than the others.The DNA sample obtained by the improved CTAB method was pure and had good quality,which could satisfy the need for further ISSR analysis.