摘要
AP2/EREBP转录因子中的RAV基因家族在植物的发育过程中发挥着重要的调控作用。利用公布的黄瓜基因组数据库CuGI,发现黄瓜基因组中存在4个RAV基因,其蛋白序列均具有完整的AP2和B3结构域;对黄瓜、拟南芥、水稻和葡萄RAV蛋白进行多序列联配,结果表明该基因家族具有高度保守性;系统发生树结合基序分析发现,进化树亚族内各成员的基序组成相似;半定量RT-PCR结果显示,4个黄瓜RAV基因的表达广泛分布于各个组织。
The RAV gene family belonging to AP2/EREBP transcription factor plays an important role in plant growth and development.Based on the published database of cucumber(Cucumis sativus L.)genome CuGI,found 4 RAV genes in cucumber genome.These 4 RAV proteins sequence have a complete AP2 and B3 domain.All RAV proteins coming from cucumber,arabidopsis,rice and grapevine were multi-sequence aligned.The results indicated that this gene family were highly conservative.Phylogenesis tree combined with motif analysis,and discovered that the motif of each RAV family within phylogenesis tree subtribe demonstrate structural similarities.The result of semi-quantitative RT-PCR analysis revealed that 4 cucumber RAV genes were expressed in multiple plant tissues.
出处
《中国蔬菜》
北大核心
2012年第08X期15-21,共7页
China Vegetables
基金
江西农业大学自由申请项目(2009)
关键词
黄瓜
RAV基因
进化分析
转录因子
基因组序列
Cucumis sativus L.
RAV gene
Phylogenetic analysis
Transcription factor
Genome sequence
