橘红色花菜薹突变体的发现和研究

Discovery and Study on Mutation Owned Orange Petal of Flowering Chinese Cabbage

以橘红色花菜薹突变体11A-47与黄色花菜薹联记特选34号甜菜心杂交获得的F1,F2及BC1、BC1′群体为试材。将6个世代的种子经4℃低温春化处理15d后调查子叶颜色,研究菜薹橘红色花的遗传规律;同时,采用与大白菜橘红心球色基因紧密连锁的分子标记对控制菜薹橘红色花的基因进行分析,鉴定菜薹橘红色花与大白菜橘红心球色基因or之间的关系。结果表明,橘红色花菜薹11A-47与黄色花菜薹杂交F2群体中,橘红色子叶与绿色子叶的分离比例符合1∶3,χ2=1.9389<χ20.05=3.841;BC1′群体中,橘红色子叶与绿色子叶的分离比例符合1∶1,χ2=1.3697<χ20.05=3.841。说明菜薹的橘红色花为质量性状,由1对隐性等位基因控制。分子标记结果表明,控制菜薹橘红色花的基因与控制大白菜橘红心球色的基因可能不同。

A F1 hybrid was obtained by crossing mutant 11A-47 with orange petal and a wild type of flowering Chinese cabbage〔Brassica campestris L. ssp. chinensis(L.)Makino var. utilis Tsen et Lee〕with yellow petal.After the population of F1,F2,BC1,BC1′ were taken as experiment materials. Seeds of 6 generations were vernalized at 4 ℃ for 15 days,and cotyledon colors were investigated to study the hereditary law of orange petal.Meanwhile,molecular markers linked to or gene in Chinese cabbage were applied to determine the characteristics of orange petal gene in flowering Chinese cabbage.Results showed that the ratio of orange with green cotyledons was 1∶3 in F2 progeny, χ2= 1.938 9 < χ20.05=3.841.The ratio of orange with green cotyledons was 1∶1 in BC1′ progeny,χ2= 1.369 7 < χ20.05=3.841.Therefore,orange petal gene in flowering Chinese cabbage is qualitative trait, and is controlled by a pair of recessive gene.Orange petal gene in flowering Chinese cabbage was probably different from gene in Chinese cabbage by molecular markers.