摘要
为探讨有机氮甘氨酸用量对生姜组培继代苗生理特性的影响,在无菌条件下以生姜组培苗为试材,设置5个不同甘氨酸浓度(1.0、1.5、2.0、2.5、3.0mg·L-1),研究了生姜组培苗氮代谢相关产物和氮代谢关键酶的活性。结果表明:生姜组培苗叶绿素含量、可溶性糖含量随着甘氨酸浓度的增加而上升,可溶性蛋白含量变化趋势则相反;谷氨酰胺合成酶和谷草转氨酶活性亦随着甘氨酸浓度的增加而升高。
In order to determine the effects of organic nitrogen glycine concentration on physiological characteristics of ginger(Zingiber officinale Rosc.)plantlet in vitro,this paper takes plantlet in vitro under aseptic cultivation as experimental material.Five kinds of glycine concentration level(1.0,1.5,2.0,2.5,3.0 mg·L-1)were set up to study the relevant product of nitrogen metabolism of ginger plantlet in vitro and the activity of nitrogen metabolism key enzyme.The results showed that the content of chlorophyll and soluble sugars raised with the increase of glycine concentration,while that of soluble protein was contraire.The activity of GS and GOT were strengthened with the increase of glycine concentration.
出处
《中国蔬菜》
北大核心
2013年第03X期54-57,共4页
China Vegetables
基金
重庆市科技创新能力建设项目(CSTC
2010CB1012)
特色植物种苗工程重庆高校创新团队建设项目(201025)
重庆高校优秀成果转化项目(Kjzh10214)
重庆市教委重点科技项目(KJ12016)
永川区科委重点攻关项目(YCSTC
2011AC1001)
关键词
甘氨酸
生姜
组培苗
生理特性
Glycine
Ginger
Plantlet in vitro
Physiological characteristics
