菜豆抗炭疽病苗期鉴定条件优化及种质资源抗性鉴定

Optimization of Conditions for Identifying Anthracnose in Phaseolus vulgaris L.Seedling Stage and Resistance Assessment of Its Germplasm Resources

对菜豆抗炭疽病苗期接种鉴定条件(产孢培养基、菌液浓度、菌种培养时间、接种位置等)进行优化,确定了豆荚培养基是最佳的产孢培养基,产孢速度快,致病力强;最佳菌液浓度为2.0×106个.mL-1,发病快,重复性好;菌种培养20d后,致病力最强,接种效果最佳;接种叶片背面感病效果最好,节省时间。同时对收集的23份菜豆材料进行苗期炭疽病抗性鉴定,其中有4份为中抗材料,1份高抗材料,抗性资源多为油豆类型。

The identification conditions including sporulation medium,bacterial concentration,culture time and inoculation position for anthracnose resistance inoculation during bean(Phaseolus vulgaris L.)seedling stage were optimized.The suitable medium was bean pod medium with rapidly spore growth and strong pathogenicity.The best concentration of vaccination was about 2.0×10 6 spores per mL,with abrupt onset and good reproducibility.After 20 days' culture,its pathogenicity was the strongest with best effect of inoculation.The best inoculation position was on the back of leave blades.It might save time.All 23 bean materials were identified for their resistance to anthracnose.Among them 4 materials were mid-resistant,1 was high resistant and most of the resistance resources were of rape bean type.