菜豆(油豆角)苗期抗炭疽病防御相关基因表达机制的初步研究

Initial Studies on Defense Gene Expression Mechanism of Phaseolus vulgaris L. var. chinensis Hort. to Anthracnose at Seedling Stage

以感病品种(老来少)和抗病品种(大油豆)为试材,在菜豆炭疽病菌侵染后的不同时间(0、3、6、9、12d)取样,采用实时荧光定量RT-PCR技术,对两者叶片中5种防御酶基因(几丁质酶、β-1,3-葡聚糖酶、SOD、POD和LOX基因)和HRGP蛋白基因相对表达量进行研究。结果表明,除β-1,3-葡聚糖酶基因、SOD基因外,其他4种防御基因在接种前的原始表达量均是抗病品种高于感病品种。接种后,感病品种中的几丁质酶基因、LOX基因表达量呈现先降低再升高的趋势,而抗病品种在感病品种表达量降到最低值(3d)时达到峰值,在整个阶段(0～12d)抗病品种的相对表达量高于感病品种;抗、感病品种的β-1,3-葡聚糖酶基因、POD基因和SOD基因的相对表达量在整个阶段呈相同的变化趋势,但抗病品种的相对表达量高于感病品种。

Use susceptible cultivar‘Laolaishao’and resistant cultivar‘Dayoudou’as test materials,the relative expression of 5 defense enzyme genes(chitinase,β-1,3-glucanase,SOD, POD and LOX gene)and HRGP protein gene were detected and analyzed by real-time PCR after 2 cultivars infected by anthracnose in different times( 0,3,6,9,12 d). The results showed that in addition to the β-1,3- glucanase gene and SOD gene,the other 4 genes expression were higher in resistant varieties than the susceptible varieties before infected;after infected chitinase gene and the LOX gene expression showed decreased-increased trend in susceptible cultivar,and resistant varieties reached the peak,when susceptible varieties decreased to the minimum(3 d),the relative expression of resistant varieties was higher than that of the susceptible ones in the whole stage(0-12 d);β-1, 3-glucanase gene,POD gene and SOD gene were in the same trend,but the expression of resistant varieties was higher than the susceptible varieties.