腐霉属菌形态学及分子生物学鉴定技术的概述

Overview of Identification Techniques for Pythium Species Based on Morphology and Molecular Biology

腐霉是一类广泛分布于世界各地的病菌。近年来,腐霉菌危害日趋明显,发生面积也逐年扩大,已从过去的次要病原菌上升为主要病原菌,给农业生产带来严重损失。本文对腐霉属菌形态学及分子生物学鉴定技术进行了综述,并提出以传统形态学为基础,利用rDNA-ITS序列、β-tubulin和CoxⅡ基因以及RFLP、RAPD、AFLP、SSR等分子标记,建立快速、准确的腐霉病害早期诊断技术是预防和控制病害发生、发展的重要策略。

Pythium are worldwide distributed oomycetes.In recent years,the plant diseases caused by Pythium become more and more obvious,the area has expanded year by year.And Pythium now regarded as a major harmful genera of plant pathogens.This paper has reviewed the identification techniques for Pythium species based on morphology and molecular biology. It also puts forward an important strategy for disease prevention and control.This strategy takes the conventional morphology as base,uses rDNA-ITS sequences,β-tubulin and CoxⅡ gene,RFLP,RAPD,AFLP and SSR,etc. molecular markers to establish a rapid and accurate diagnosis technology.