西瓜遗传图谱的构建及果形指数QTL分析

Construction of Watermelon Genetic Map and Fruit Shape Index QTL Analysis

以圆球形西瓜品系花园母本为母本,椭圆形西瓜品系LSW-177为父本,配制杂交组合,构建由180个单株组成的F2群体,测量成熟西瓜的果实纵径、果实横径,并且通过果实的纵径与横径的比值得到果形指数,进行遗传图谱的构建及相关性状QTL分析。构建的连锁遗传图谱共含有45个SSR标记,分属13个连锁群。覆盖基因组长度为547.3cM,标记间平均距离为12.44cM,检测到与果形指数密切相关的QTL1个,并在遗传连锁图谱中将之命名为Fsi3.1,位于第三连锁群,位于标记MCPI-12和CMN05_69之间,贡献率为2.8%,加性效应负值,对减小果形指数表现为增效加性效应。

Taking round shape watermelon line‘Huayuanmuben’as female parent and oval shape line‘LSW-177’as male parent,we crossed them and obtained F2 population constructed with 180 single plants.We measured the length and width of the ripened watermelon〔Citrullus lanatus (Thunb.)Matsum. et Nakai〕,obtained fruit shape index from the ratio between fruit length and width, constructed the genetic map and analyzed the QTLs of relevant characteristics.The constructed genetic map contains 45 SSR-markers belonging to 13 linkage groups.The covering genome length was 547.3 cM.The average distance between markers was 12.44 cM.The one QTL closely associated with fruit shape index was located in the 3rd linkage group between MCPI-12 and CMN05_69.This QTL was named as Fsi3.1.Its contribution rate was 2.8%,and its additive effect was negative,which showed synergia additive effect in reducing fruit shape index.