甜瓜种质资源遗传多样性的SSR分析

Analysis of Melon Germplasms Genetic Diversity by SSR Markers

利用SSR分子标记技术对72份甜瓜种质资源及育种材料进行DNA指纹分类及亲缘关系研究,20对SSR引物共扩增产生76个多态性位点,平均每对引物产生3.8个多态性位点。聚类结果将72份甜瓜种质资源分为7个类群,其中类群I包括新疆本地资源及几乎全部自育甜瓜种质资源64份材料,这说明本试验所选用的新疆厚皮甜瓜种质资源遗传背景比较狭窄。

SSR molecular marker technique was used to assess the DNA fingerprint classification and genetic relationship of 72 melon germplasm resources and breeding materials. From 20 pairs of SSR primers, 76 polymorphic bands were generated. On average each pair of primers produced 3.8 bands.The clustering result divided the 72 melon germplasm resources into 7 groups.There were 64 accessions in guoup I, including Xinjiang local resources and almost all of the self bred melon germplasm resources.This means that the genetic background of Xinjiang muskmelon germplasm resources is comparatively narrow.