氯化胆碱诱导黄瓜抗白粉病机理研究

Studies on Cucumber Resistance Mechanism to Powdery Mildew Induced by Choline Chloride

氯化胆碱是一种新型广效的生长调节剂,以10mmol·L-1氯化胆碱处理黄瓜幼苗可明显提高植株的株高、根长、鲜质量、干质量和叶绿素含量。诱导抗性机理研究表明:氯化胆碱处理后,黄瓜幼苗体内β-1,3-葡聚糖酶、几丁质酶活性及富含羟脯氨酸糖蛋白、丙二醛含量均明显上升。以白粉病菌分生孢子接种经氯化胆碱处理过的黄瓜叶片,结果显示分生孢子萌发率较低,芽管较短,且新产生的分生孢子串数量较少。用氯化胆碱处理黄瓜植株,可延迟和减轻黄瓜白粉病的发生。

Choline chloride is a new growth regulator. When the cucumber seedlings were treated with 10 mmol·L-1 choline chloride,their plant height,root length,dry weight,fresh weight,and chlorophyll content were remarkably improved.Studies on induce resistance mechanism showed that the content or activity of β-1,3-glucanase,chitinase,hydroxyproline rich glycoproteins(HRGP)and malondialdehyde(MAD)of cucumber seedlings were significantly increased after being treated with choline chloride.The conidiospores of Sphaerotheca fuliginea were inoculated on the cucumber leaves treated with choline chloride.The results showed that the germination rate of conidiospores was decline. The length of germ tubes was shorter and the number of new conidia strings was less than the contrast. Choline chloride treatment on cucumber plants can delay and alleviate the occurrence of powdery mildew.