黄瓜低农药残留品种的外果皮组织解剖学特征比较分析

Comparative Analysis of Epicarp Tissue Anatomical in Low Pesticide Residue Cucumber Strain

以黄瓜低农药残留品系D0351及其相对应的高农药残留品系D9320作为材料,在始收期分别喷施霜霉威和毒死蜱两种农药,对两个品系外果皮细胞的形态、气孔、叶绿体以及线粒体超微结构进行比较分析。结果表明:在自然状态下,两个品系的最大差异是外果皮细胞结构,D0351品系外果皮细胞呈长条形,D9320品系呈近正方形,D0351品系的外表皮细胞面积小于D9320品系。两个品系经农药处理后,D0351外果皮细胞变成近圆形或长圆形,D9320外果皮细胞形态没有改变,两个品系的外果皮细胞面积都增加,且D0351的细胞面积仍小于D9320;气孔开口度D0351的增大程度比D9320小;两个品系的叶绿体内的嗜锇颗粒数量变多、体积变大;线粒体形态及内部结构没有改变。

The low pesticide residue cucumber(Cucumis sativus L.)strain(D0351)and the high pesticide residue strain(D9320)were taken as tested materials.Chlorpyrifos and propamocarb pesticide were sprayed on cucumber plants in harvest beginning stage.The epicarp cells morphology, stoma,chloroplast and mitochondria ultra-structure of these 2 strains were compared.The result indicated that the biggest difference between these 2 strains was the structure of the epicarp cells.The epicarp cell of strain D0351 was elongated,while that of strain D9320 was foursquare.The outer area of the epicarp cell of D0351was smaller than that of D9320.After pesticide sprayed treatment,the epicarp cells of the strain D0351 became round or oblong,and the epicarp morphology of strain D0351 was changed,while that of strain D9320 was not changed.The epicarp cell areas of both strains were increased.As for the stoma opening degree the strain D9320 is bigger than strains D0351.The number of osmium particle of both strains were increased and their sizes became larger in the chloroplast. Mitochondrial morphology and internal structure was not changed.