摘要
为明确豫西地区茄科作物土壤镰孢菌菌株间的系统发育关系,对从豫西地区17个县、市采集的32份土壤样品中分离得到的96株镰孢菌进行形态学鉴定及EF-1α序列分析。结果表明:供试镰孢菌分属于5个种,位于系统发育树的5个分支上,分别为尖孢镰孢菌(F.oxysporum)、藤仓镰孢菌(F.fujikuroi)、轮枝镰孢菌(F.verticillioide)、F.incarnatum和F.incarnatum-equiseti species complex。其中尖孢镰孢菌(F.oxysporum)的分离频率最高,为45.83%,在所调查的4种茄科作物土壤中均有分布;而F.incarnatum-equiseti species complex的分离频率最低,仅在马铃薯和番茄土壤中存在。不同地区茄科作物土壤镰孢菌种群存在差异。
Morphological observation and sequence analysis of EF-1α of testedFusarium strains collected from the Solanaceae crops soil in western Henan Province were carried out to clarify the species diversity and phylogenetic relationships amongFusarium strains.The results showed that 5 branches ofFusarium were identified in the phylogenetic tree,namelyF. oxysporum,F. fujikuroi,F. verticil-lioide,F. incarnatum andF.incarnatum-equiseti species complex. Among them,F. oxysporum had the highest separation frequency of 45.83%,and was distributed in all investigated 4 kinds of Solanaceae crops soil.While,the separation frequency ofF.incarnatum-equiseti species complex was the lowest, it only existed in potato and tomato soils.In addition,species diversity existed inFusarium strains from different regions and different Solanaceae crops soil.
出处
《中国蔬菜》
北大核心
2013年第12X期65-69,共5页
China Vegetables
基金
河南省基础研究计划项目(122300410051)
洛阳师范学院青年科研基金项目(2011-QNJJ-004)
