UL16结合蛋白1在子痫前期孕妇外周血外泌体中表达的分析

Analysis of unique long 16 binding protein 1 expression in peripheral blood exosomes of pregnant women with preeclampsia

目的探讨UL16结合蛋白1(ULBP1)在子痫前期(PE)患者外周血外泌体中的表达情况。方法选取2017年6月至2018年7月就诊于中国医科大学附属第一医院产科的PE患者(PE组)20例,正常对照组(NC组)为健康孕产妇20例,均为初产妇、单胎,留取空腹肘正中静脉血液标本。通过低速离心分离出血清,使用ExoQuick Precipitation kit试剂盒提取血清中的外泌体,采用蛋白免疫印迹实验(Western bolt)检测外泌体的标志蛋白CD63,使用透射电镜鉴定外泌体的形态,通过免疫酶联吸附试验(ELISA)检测外泌体中ULBP1的水平。结果 ULBP1在PE组外周血外泌体中的水平[(1.0500±0.5937)μg/L]高于NC组[(0.4875±0.4346)μg/L],差异具有统计学意义(P=0.0013)。结论 PE患者外周血外泌体中ULBP1的表达升高,其升高可能会为PE的诊治提供一定依据。

Objective To investigate the expression of unique long 16 binding protein 1(ULBP1)in peripheral blood exosomes of patients with preeclampsia(PE).Methods Twenty patients with PE who were admitted to the First Affiliated Hospital of China Medical University from June 2017 to July 2018 were enrolled.The normal control group(NC group)consisted of 20 normal pregnant women,all of whom were primiparas,singleton.Blood samples were taken from the median vein of the fasting elbow.The serum was separated by low-speed centrifugation.Extraction of exosomes from serum was performed using ExoQuick Precipitation kit,and the exosome marker protein CD63 was detected by Western blot,the morphology of exosomes were identified by transmission electron microscopy,and the expression of ULBP1 in exosomes was detected by enzyme-linked immunosorbent assay.Results The expression of ULBP1 in peripheral blood exosomes was significantly higher in PE than in the control group[(1.0500±0.5937)μg/L vs.(0.4875±0.4346)μg/L,P=0.0013(P<0.05)].Conclusion The expression of ULBP1 in peripheral blood exosomes of PE patients is increased,and its increase may provide a basis for the diagnosis and treatment of PE.