有氧运动联合破壁蛋白核小球藻对高脂膳食大鼠脂代谢某些指标的影响

Effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on some indicators of lipid metabolism in rats with high-fat diet

目的:研究有氧运动联合破壁蛋白核小球藻对高脂膳食大鼠脂代谢某些指标的影响。方法:55只雄性Wistar大鼠经过适应性饲养4 d后进行3 d、20 min/d的无负重游泳训练,筛选淘汰5只不适应游泳训练的大鼠后,按体重以数字随机分组法分为5组:普通膳食+安静对照组(C组)、高脂膳食+安静对照组(H组)、高脂膳食+小球藻+安静对照组(HC组)、高脂膳食+有氧运动组(HM组)、高脂膳食+有氧运动+小球藻组(HMC),每组10只。HM组和HMC组进行6周每周6次60 min/d的无负重游泳训练。C组大鼠以普通饲料常规喂养;其余各组以高脂饲料喂养; HC组和HMC组大鼠灌胃破壁小球藻1次,灌胃剂量为3.9 g/(kg·d),灌胃体积为5 ml/kg,其余各组ig等量生理盐水。6周后,测定大鼠Lee’s指数,取血、肝脏检测相关生化指标。结果:与C组比较,H组Lee’s指数,血清FFA、IL-6、TNF-α、TC、TG、LDL-c,肝脏FFA、IL-10显著升高(P<0.01);血清HDL-c水平显著降低(P<0.01)。与H组比较,HC、HM、HMC组Lee’s指数,血清FFA、IL-6、TNF-α、TC、TG、LDL-c,肝脏FFA、IL-10显著降低(P<0.05或P<0.01);血清HDL-c水平显著升高(P<0.05或P<0.01)。与HC、HM组比较,HMC组Lee’s指数,血清FFA、IL-6、TNF-α、TC、TG、LDL-c,肝脏FFA、IL-10显著降低(P <0. 05);血清HDL-c水平显著升高(P <0. 05)。结论:有氧运动和破壁蛋白核小球藻干预能够不同程度改善高脂膳食大鼠脂代谢,降低肥胖对肝脏造成的脂毒性。其中联合干预较单一干预更为有效。

Objective: To study the effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on the lipid metabolism in rats with high-fat diet. Methods: Fifty-five male Wistar rats were subjected to adaptive feeding for 4 days and weight-free swimming training for 3 days,20 min/d. After eliminating 5 rats that were not suitable for swimming training,the other rats were randomly divided into 5 groups according to their body weight: control group( C group),high fat diet group( H group),high-fat diet + chlorella group( HC group),high fat diet + aerobic exercise group( HM group),high fat diet + chlorella + aerobic exercise group( HMC group),10 in each group. The HM and HMC group were subjected to 60 min/d swimming training for 6 weeks with nonweight-bearing. Group C were fed regular diet. The other groups were fed with high-fat diet,the rats in group HC and HMC were intragastrically treated with chlorella pyrenoidos of disintegrated cell wall at the dose of 3.9 g/( kg·d),the volume was 5 ml/kg,and the other groups are given equivalent saline. The Lee’s index and biochemical indexes of blood and liver were measured after 6 weeks. Results: Compared with group C,Lee’s index,serum levels of free fatty acids( FFA),interleukin-6( IL-6),tumor necrosis factor-α( TNF-α),total cholesterol( TC),triglyceride( TG),low density lipoprotein cholesterol( LDL-c),liver FFA and interleukin-10( IL-10) were increased significantly( P<0.01),the serum level of high-density lipoprotein cholesterol( HDL-c) was decreased significantly( P<0.01) in group H. Compared with group H,Lee’s index,serum FFA,IL-6,TNF-α,TC,TG,LDL-c,liver FFA and IL-10 were decreased significantly( P<0.05 or P<0.01),serum level of HDL-c was increased significantly( P< 0.05 or P< 0.01) in group HC,HM and HMC. Compared with group HC and HM,Lee’s index,serum FFA,IL-6,TNF-α,TC,TG,LDL-c,liver FFA and IL-10 were decreased significantly( P<0.05),serum level of HDL-c was increased significantly( P<0.05) in group HMC. Conclusion:Aerobic exercise and chlorella pyrenoidos of disintegrated cell wall can improve lipid metabolism in rats with high-fat diet and reduce the lipid toxicity caused by obesity. Joint intervention is more effective than single intervention.