灰霉菌侵染对蓝莓采后品质变化及抗氧化性的影响

Effects of Botrytis cinerea Infection on Post-harvest Quality and Antioxidant Activities in Blueberry Fruits

灰霉病是蓝莓采后高发病害之一,严重影响贮藏品质。以"灿烂"蓝莓为材料,以无菌水处理的果实为对照,研究灰葡萄孢霉侵染对蓝莓采后品质和抗氧化性的影响。结果表明:受侵染的蓝莓果实在贮藏前期活性氧自由基(ROS)迅速积累,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性提高,同时抗坏血酸、谷胱甘肽等抗氧化物质含量增加;贮藏后期抗氧化酶活和抗氧化物质含量下降,活性氧清除能力不足导致ROS过度积累,细胞膜结构破坏,加速了蓝莓的成熟、软化、衰老和腐烂。

Gray mold is one of the frequently-occurring postharvest diseases of blueberry, which seriously affects the storage quality. The effects of Botrytis cinerea on the postharvest quality and antioxidant system of ’Brilliant’ blueberry were studied with the fruits treated with sterile water as the control. The results showed that the reactive oxygen species(ROS) accumulated rapidly, the activities of superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX),glycopeptide reductase(GR) and the the contents of ascorbic acid and glutathione increased rapidly in the infected blueberry fruits. At the late storage stage, the activities of antioxidant enzymes and antioxidant level were decreased, and the insufficient scavenging capacity of ROS lead to excessive accumulation of ROS and destruction of cell membrane structure, which accelerates the ripening, softening, aging and decay of blueberries.