科尔沁沙质草地纤维素分解菌的筛选、鉴定及其分解能力

Isolation and Identification of Cellulose Decomposing Fungi and Their Decomposition Ability in the Horqin Sandy Grassland

从科尔沁沙质草地土壤中,通过分离、纯化、初筛和复筛得到2株分解纤维素(CMC)能力较强的真菌(NM1-1和NM1-2),采用形态学和rDNA-ITS分子生物学相结合的方法鉴定分别为伪弯头曲霉(Asperigillus pseudodeflectus)和尖孢镰刀菌(Fusarium oxysporum)。NM1-1和NM1-2对布条的分解效果较好,30d的分解率分别达到15.69%和16.91%;凋落物分解试验表明,加NM1-1和NM1-2的草地凋落物分解率在30d内分别达53.61%和45.72%,分别比对照高7.70和6.42倍。NM1-1和NM1-2的CMC酶活性显著高于其生存的土壤环境。科尔沁沙质草地土壤高效纤维素分解菌的分离和筛选不仅补充了沙质草地土壤功能微生物库,同时对沙地凋落物分解和土壤有效养分输入起到促进作用。

Two effective cellulose decomposing fungi(NM1-1 and NM1-2)were isolated and screened in the Horqin Sandy Grassland.They were identified as Asperigillus pseudodeflectus and Fusarium oxysporum by morphological and rDNA-ITS molecular biological methods.Cloth decomposition rate affected by NM1-1 and NM1-2 were 15.69% and 16.91% in 30 days,respectively;and litter decomposing rate affected by NM1-1and NM1-2 were 53.61% and 45.72%in 30 days,and the rates were 7.70 and 6.42 times higher than that without any microorganism,respectively.The CMC enzyme activity was significantly higher produced by NM1-1 and NM1-2 than the soil environment where they were isolated.Isolation and screening of effective cellulose decomposing fungi supplemented the soil functional microbe bank,accelerate litter decomposing rate and stimulate available nutrient input in the Horqin Sandy Grassland.