马立克病抗性选育对鸡感染马立克病病毒后外周血淋巴细胞病毒载量的影响

Effects of Marek's disease resistance selection on virus loads in peripheral blood lymphocytes of chickens challenged with Marek's disease virus type 1

为研究马立克病(MD)抗性选育对鸡马立克病病毒(MDV1)感染后外周血淋巴细胞病毒载量的影响,利用MDV1攻毒后第4、7、10、14、21、28和35天共7次采集并分离的全部试验鸡的外周血淋巴细胞为材料,采用real-time FQ-PCR方法对这些材料中meq基因进行绝对定量分析以确定其病毒载量,用来分析比较MDV1在MD抗性鸡与普通鸡以及HVT疫苗免疫鸡与HVT疫苗未免疫鸡体内的增殖情况。结果显示,普通鸡非免疫攻毒组(B组)的MDV1含量于攻毒后第10(P<0.05)、14(P<0.05)、21(P<0.01)、28(P<0.05)、35(P<0.05)天显著高于MD抗性鸡免疫攻毒组(F组),而其他组间均无显著性差异。结果表明,鸡MD抗性选育结合HVT疫苗接种可显著降低鸡体内的病毒载量,从而降低MDV1感染的危害。

Effects of Marek's disease(MD)resistance selection on virus loads in peripheral blood lymphocytes(PBLs)of chickens after the challenge with Marek's disease virus type 1(MDV1)were investigated.Real-time FQ-PCR was used for absolute quantification of the DNA levels of meq gene of PBLs sampled on 7different time points(on day 4,7,10,14,21,28and 35post-infection)from all birds after the challenge of MDV1.The load of MDV1was detected in PBLs to analyze and compare proliferation of MDV1in resistant/common or/and HVT-vaccinated/ HVT-unvaccinated birds.In result,the load of MDV1 was significantly higher in chickens of B line(common line,HVT-unvaccinated and MDV1-challenged)but significantly lower in chickens of F line(MD-resistant line,HVT-vaccinated and MDV1-challenged)on day10(P<0.05),14(P<0.05),21(P<0.01),28(P<0.05)and 35(P<0.05)post-infection.There was no significant difference between the other groups.In conclusion,virus loads in chickens can be reduced significantly by resistance selection in coordination with vaccination,which can reduce the harm of MDV1infection.