成年牦牛几种主要组织细胞中胞红蛋白的分布

Distribution of cytoglobin in several major tissues and cells of adult yak

为了探讨胞红蛋白(CYGB)在成年牦牛不同组织细胞中的分布特征,选择健康成年牦牛8只,利用免疫组织化学染色SP法观察CYGB在成年牦牛不同组织细胞中的分布特征。结果显示,CYGB在成年牦牛大脑和小脑皮质部与髓质部、脊髓、肾上腺皮质部、垂体、松果体、胰岛、睾丸、附睾、卵巢、子宫、胃、肠、肝、唾液腺、心肌、骨骼肌、脾、肺和肾等组织细胞中均有表达,CYGB免疫阳性物质主要定位于细胞质。此外,在牦牛皱胃胃底腺、小肠腺以及十二指肠腺分泌细胞中也发现有CYGB阳性表达。在肾上腺髓质部和肾的肾小球内未见CYGB阳性表达。结果表明,CYGB在成年牦牛多种组织细胞中广泛分布,CYGB在这些组织的氧利用及功能活动中发挥作用。

To explore the localization of cytoglobin(CYGB)in adult yak tissues and cells,eight healthy adult yaks were used in this study.CYGB expression in adult yak tissues and cells was localized using the immunohistochemical SP technique.In result,CYGB-positive cells were distributed in the tissues and cells of the brain and cerebellum cortex and medulla,spinal,adrenal cortex,pituitary,pineal glands,islets,testis,epididymis,ovary,uterus,stomach,intestines,liver,salivary glands,heart,skeletal muscle,spleen,lung and kidney.CYGB was mainly localized in cytoplasm.Besides,the CYGB-positive cells were found in the fundic glands of the abomasum,intestinal glands and duodenal glands.CYGB-negative cells were found in the adrenal medulla and kidney glomerulus.The widely expression of CYGB in various tissues and cells in adult yak suggested that CYGB might play a role in the utilization of oxygen and physiological functions.