摇晃蛋白对小鼠海马MFS发育影响的双光子显微成像研究

Effects of reelin on development of mossy fiber sprouting of mice using two-photon imaging system

为了掌握小鼠大脑发育过程中摇晃蛋白(reelin)与颗粒细胞苔藓纤维出芽(mossy fiber sprouting,MFS)的关系,通过双光子显微镜扫描转基因小鼠Thy1-GFP和Reln-Thy1-GFP的脑片,观察位于海马分子层的颗粒细胞苔藓纤维的出芽和发育情况,统计分析野生型小鼠和摇晃小鼠脑片在不同培养时期苔藓纤维出芽的数量和体积。结果显示,Thy1-GFP转基因小鼠的海马脑片是观察颗粒细胞苔藓出芽和发育的良好材料;野生型小鼠和摇晃小鼠海马苔藓纤维出芽的数量和体积随发育时间的延长有明显的动态变化。结果表明,摇晃蛋白能够促进颗粒细胞苔藓纤维出芽并参与轴突的发育。

To understand the effect of reelin on mossy fiber sprouting by means of two-photon microscopy in research of transgenic mice slice culture,the mossy fiber sprouting development in molecular layer of granule cells was observed.Quantitative analysis was carried out on mossy fiber sprouting of a dentate granule cell over time.In result,two-photon laser scanning microscopy offers a reliable method for further studies of fine structures in Thy1-GFP mice.The numbers of mossy fiber sprouting increased and the volume were very dynamic with an increase over time.In conclusion,reelin can promote the mossy fiber sprouting of granule cells and participate in the development of neuron axon.