犬瘟热病毒感染小鼠脾淋巴细胞所引起的主要基因表达情况的变化

Gene expression profile of murine splenic lymphocytes infected with canine distemper virus

采用基因芯片技术对正常小鼠淋巴细胞和犬瘟热病毒(CDV)感染后第7天的小鼠淋巴细胞基因表达的差异性进行了比较,从分子水平上探究了CDV感染机体后的免疫应答情况。结果显示,检测到364个差异表达基因,其中280个基因的表达水平升高,84个基因的表达水平下降。进一步以基因芯片中CDV感染后上调基因C-C型趋化因子受体2(CCR2)和下调基因CD14为研究对象,应用实时荧光定量方法验证基因芯片的结果,两者完全相符,说明芯片的筛查结果可靠。根据基因芯片的结果,采用Go注释系统和数据库查询,对364个差异表达基因进行功能注释,结果显示,差异表达基因主要与水解、结合、转运调控、信号传递、代谢、结构组成等有关,其中CCR2基因、CD14基因、丝裂原活化蛋白激酶1(MAP3K1)基因、CD69基因等被鉴定为差异表达基因。上述结果为进一步阐明CDV感染后机体的免疫应答机制奠定了基础。

The differentially-expressed genes of murine splenic lymphocytes following canine distemper virus(CDV)infection on day 7post-infection were analyzed by cDNA microarray.A total of 364differentially-expressed genes including 280up-regulated and 84down-regulated genes were identified.Two differentially-expressed genes chemokine(C-C motif)receptor 2(CCR2)gene and CD14 gene were selected to validate the reliability of cDNA microarray by quantitative real-time PCR(qRT-PCR)and the result of qRTPCR was consistent with cDNA microarray.The function of the differentially-expressed genes was annotated by GO Annotation System according to the results of cDNA microarray.In result,the differentiallyexpressed genes were mainly involved in the processes of proteolytic reactions,binding,transcriptional regulation,signal transmission,metabolism,and structural composition.Among them,CCR2 gene,CD14gene,mitogen-activated protein kinase kinase kinase 1(MAP3K1)gene and CD69 gene were identified as differentially-expressed genes.Therefore,the above-mentioned results will provide a platform to explore molecular mechanisms of immune responses of CDV infection.