核因子-κB在滇南小耳猪胆道损伤修复模型中表达的研究

Study of NF-κB expression in diannan small-eared pig's bile duct injury model

目的分析核因子-κB(nuclear factor,NF-κB)及其下游抗凋亡caspase-8同源结构FLICE抑制蛋白(cFLIP)在胆道瘢痕中的表达情况。方法建立滇南小耳猪胆道损伤修复模型并建立对照组。用免疫组化分析NF-κB及cFLIP的表达范围及强度。结果和对照组相比,胆道瘢痕组织中NF-κB及cFLIP表达的范围更广,强度更强,更多的集中于成纤维细胞中,且两者表现出正相关关系。结论胆道损伤后NF-κB激活增加并导致cFLIP蛋白过表达,可能导致胆道成纤维细胞凋亡减少,从而导致胆道瘢痕的形成。

Objective To analyze expression of NF-κB and it s downstream anti-apoptosis cFLIP protein in bile duct stricture.Methods Build diannan small-eared pig' s bile duct repair injury model and contrast group.Use immunohistochemical method analyses NF-κB and cFLIP expressive scope and density,and compared with contrast group.Results NF-κB and cFLIP in bile duct s scar organization expressive scope is wider and density is stronger than normal bile duct organization.NF-k B and cFLIP specially concentrate in fibroblast and accord to positive correlation.Conclusion cFLIP over-expressed caused by NF-K B activation after bile duct injury probably reduce fibroblast's apoptosis and augment proliferation.As result,improving formation of bile duct scar.