强直性脊柱炎患者咽部菌群变化

The changes of pharyngal microbiota in patients with ankylosing spondylitis

目的探讨强直性脊柱炎患者的咽部菌群变化。方法筛选入组7例强直性脊柱炎患者和7例健康者咽拭子样本,提取咽部DNA,扩增16SrRNA基因,在Illumina平台测序,对测序结果进行生物信息学分析。结果从ACE指数、Chao1指数、Shannon指数和Simpson指数综合来看强直性脊柱炎患者的咽部菌群Alpha多样性差异不大。Beta多样性分析显示两组研究对象咽部菌群样本可被区分。强直性脊柱炎患者咽部菌群组成和含量发生显著改变,主要变化包括:拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria)显著降低。拟杆菌门中普雷沃杆菌属(Prevotella)相关的纲目科属水平都显著降低。放线菌门变化落实到属水平,放线菌属(Actinomyces)显著降低,丙酸杆菌属(Propionibacterium)和棒状杆菌属(Corynebacterium)显著增高。厚壁菌门(Firmicutes)中,芽胞杆菌纲(Bacilli)所属的与链球菌属(Streptococcus)相关的纲目科属水平显著增加,而梭状芽胞杆菌纲(Clostridia)包含的韦荣球菌属(Veillonella)、消化球菌属(Peptococcus)显著下降。此外,变形菌门中出现弧菌属(Vibrio)的增加和弯曲菌属(Campylobacter)的降低等变化。结论强直性脊柱炎患者(本次研究样本)的咽部菌群出现紊乱,以普雷沃杆菌属、放线菌属、韦荣球菌属、消化球菌属和弯曲菌属等显著降低,丙酸杆菌属、棒状杆菌属、链球菌属和弧菌属等显著增加为主要特征。

Objective To investigate the changes of pharyngal microbiota in patients with ankylosing spondylitis(AS).Methods Seven AS patients and seven healthy controls were recruited in this cross-sectional study,the pharyngal microbiota status between two groups were analyzed after extracting pharyngal microbiota DNA and sequencing on Illumina platform of bacterial 16 SrRNA.Results The Alpha diversity of pharyngal microbiota in patients with AS was not significantly different from the ACE index,Chao1 index,Shannon index and Simpson index.The Beta diversity analysis showed that the two groups of pharyngal microbiota samples could be distinguished.The composition and content of pharyngal microbiota in patients with AS were significantly changed.Major changes included:a significant decrease in Bacteroidetes and Actinobacteria.The level of class,order,family,genus related to Prevotella decreased significantly in Bacteroidetes.At the level of genus,Actinomyces had been significantly decrease,Propionibacterium and Corynebacteriumhad been significantly increase in Actinobacteria.The level of class,order,family,genus related to Streptococcus belong to Bacilli in Firmicutes showed a significant increase,while the level of the genus related to Veillonella,Peptococcus belong to Clostridia showed a significant decrease.In addition,Vibrio increased and Campylobacterdecreased in Proteobacteria.Conclusion The pharyngal microbiota of patients with AS was disturbed.The characteristic of pharyngal microbial dysbiosis in this study:the level of Prevotella,Actinomyces,Veillonella,Peptococcus,Campylobacter were significantly reduced while the level of Propionibacterium,Corynebacterium,Streptococcus and Vibrio were significantly increased.