摘要
目的探讨广东韶关地区女性人乳头瘤病毒(HPV)感染现状及病毒基因型分布情况。方法采用PCR-反向点杂交技术对54 597例女性宫颈脱落细胞标本进行23种HPV基因分型检测。结果 54 597例女性中共检出HPV阳性13 824例,阳性率为25.32%。全部标本中高危型感染占19.91%(10870/54597),主要包含HPV52 (2187/54597)、HPV16 (1852/54597)、HPV58 (1226/54597)、HPV53(801/54597)和HPV68 (729/54597)。低危型感染占5.41%(2954/54597),主要包含HPV43 (896/54597)和HPV81 (833/54597)。感染类型中单一感染占79.22%(8545/10787),二重感染占15.62%(1685/10787),三重及以上感染占5.16%(557/10787)。结论韶关地区女性HPV感染率较高,且以高危型和单一型感染为主。病毒主要基因型为HPV52、HPV16和HPV58。进行HPV基因分型检测对宫颈癌的防治和疫苗研发接种具有重要意义。
Objective To investigate the infection status and genotype distribution of papillomavirus(HPV)in females in Shaoguan,Guangdong.Methods PCR-reverse dot hybridization was used to detect 23 HPV genotypes in 54,597 female cervical exfoliated cells.Results A total of 13,824 HPV positive cases were detected in 54,597 females.The positive rate was 25.32%(13824/54597).Among them,high-risk HPV genotype infections accounted for 19.91%(10870/54597),mainly including HPV52(2187/54597),HPV16(1852/54597),HPV58(1226/54597),HPV53(801/54597)and HPV68(729/54597).Low-risk HPV genotype infections accounted for 5.41%(2954/54597),mainly including HPV43(896/54597)and HPV81(833/54597).Single infections,double infections,triple and multiple infections respectively accounted for79.22%,15.62%and 5.16%.Conclusion The rate of HPV infection is high in females in Shaoguan.The main infections are high-risk and single-type HPV infections.The main genotypes are HPV52,HPV16 and HPV58.The detection of HPV genotypes is of great significance for the prevention and treatment of cervical cancer and vaccine research and vaccination.
作者
马占忠
许红雁
胡红波
何凤屏
邢伟斌
刘玉兰
MA Zhanzhong;XU Hongyan;HU Hongbo;HE Fengping;XING Weibin;LIU Yulan(Yuebei People′s Hospital of Shantou University,Shaoguan,Guangdong 512026,China)
出处
《中国微生态学杂志》
CAS
CSCD
2019年第3期335-338,共4页
Chinese Journal of Microecology
关键词
人乳头瘤病毒
基因型
韶关
Human papillomavirus
Genotype
Shaoguan