地衣芽胞杆菌BL63516对抗生素诱导的菌群紊乱小鼠肠黏膜屏障功能的影响

Effects of Bacillus licheniformis BL63516 on the intestinal mucosal barrier function of mice with antibiotic-induced dysbiosis

目的探索地衣芽胞杆菌BL63516对肠道菌群和肠黏膜屏障功能调节的效果和可能机制,为临床疾病的治疗提供新思路。方法采用健康清洁级雄性BALB/C小鼠30只构建动物模型,将小鼠分为3组(每组10只),分别为对照组(CON组)、头孢曲松钠灌胃组(CS组)和地衣芽胞杆菌BL63516治疗组(BL组),除CON组外其余两组小鼠给予300 mg/mL头孢曲松钠0.2 mL,2次/d灌服8 d,短期大量服用抗生素以建立肠道菌群紊乱模型。第9天起BL组小鼠给予地衣芽胞杆菌干预连续8 d。第17天摘眼球处死小鼠,取血、结肠、粪便样本。ELISA方法检测小鼠血清及肝脏组织中的细胞因子及LPS水平。PCR-变性梯度凝胶电泳方法及16S rRNA高通量测序测定小鼠肠道菌群结构。结果地衣芽胞杆菌干预后小鼠血清LPS水平较模型组显著降低,屏障功能改善。且地衣芽胞杆菌治疗后BL组小鼠肠道菌群丰富度和多样性显著升高,菌群结构有所改变。结论实验结果提示地衣芽胞杆菌BL63516可以有效改善抗生素所致的肠道菌群失调,对肠屏障功能有一定调节作用。

Objective To explore the effects of Bacillus licheniformis BL63516 on intestinal microbiota and the function of intestinal mucosal barrier,and provide new ideas for the treatment of clinical diseases.Methods Thirty mice were divided into control group(CON group),ceftriaxone sodium group(CS group)and Bacillus licheniformis group(BL group)respectively.The mice in CS group and BL group were fed 300 mg/mL ceftriaxone sodium,bid for 8 days,and then gavaged with Bacillus licheniformis and xylo-oligosaccharide separately from the ninth day.The mice were killed on the 17 th day to collect blood,colon and feces for detection of the levels of serum cytokines and LPS by using ELISA.The structure of gut flora was determined using PCR-DGGE and 16 S rRNA high-throughput sequencing.Results Compared with the CS group,the level of serum LPS in BL group significantly decreased.The function of intestinal mucosal barrier in BL group was improved due to the treatment with Bacillus licheniformis.Both the abundance and diversity of gut microbiota in BL group significantly increased.Conclusion Bacillus licheniformis BL63516 can effectively improve the gut microbiota disorder and intestinal barrier function injury caused by antibiotics.