邻苯二甲醛对细菌芽孢和分枝杆菌杀灭效果研究

Study on disinfection effect of Ortho-phthalaldehyde on bacteria spores and mycobacterium

目的观察某邻苯二甲醛消毒液对细菌芽孢和分枝杆菌的杀灭效果及连续使用稳定性。方法采用悬液定量杀菌试验,对该邻苯二甲醛消毒液杀灭枯草杆菌黑色变种芽孢和龟分枝杆菌脓肿亚种的效果进行观察。结果浓度为4 800 mg/L的该消毒液作用14 h,对龟分枝杆菌脓肿亚种作用5 min杀灭对数值为4. 60;浓度为3 000 mg/L的该邻苯二甲醛消毒液的稀释液作用14 h,对悬液内枯草杆菌黑色变种芽孢的杀灭对数值为7. 65,杀灭对数值为4. 02,作用5 min对龟分枝杆菌脓肿亚种的杀灭对数值仅为2. 88。该消毒液经医疗器械浸泡连续使用14 d的稳定性实验结果证明,杀菌效果不变。该消毒液在内镜清洗机内连续使用14 d共处理80条模拟内镜后,杀菌效果未达到高效消毒要求。结论该邻苯二甲醛消毒液所设定的剂量不适用于医疗器械的高水平消毒。

Objective To study the disinfection effect of Ortho-phthalaldehyde( OPA) on bacteria spores and mycobacterium,and to evaluate the stability of the OPA during continuous use. Methods The germicidal effect of OPA on Bacillus subtilis var. niger spores and Mycobacterium chelonei subsp. abscessus were evaluated by suspension quantitative bactericidal test. Results When using 4 800 mg/L OPA to treat B. subtitle var. niger spores for fourteen hours and M. chelonei subsp.abscessus for 5 mins,the killing log value was 7. 65 and 4. 60 respectively. When using 3 000 mg/L OPA to treat B. subtitle var. niger spores for 14 hours and M. chelonei subsp. abscessus for 5 mins,the killing log value was 4. 02 and 2. 88 respectively. The germicidal effect of OPA was stable when continuously being used for immersion of instruments for 14 days. While the germicidal effect could not reach high level disinfection effect when continuously being used in endoscopic cleaning machine for 14 days,with the total number of treated endoscope of 80 strips. Conclusion The working concentration offered by manufacturer is not appropriate for high level disinfection of medical instrument.