一种复方苯扎氯铵卫生湿巾的杀菌效果和毒性观察

The germicidal efficacy and toxicity of a kind of compound benzalkonium chloride wet tissue

目的观察一种复方苯扎氯铵卫生湿巾的杀菌效果和毒性。方法采用载体定量杀菌试验和动物毒性评价试验,对一种含100 mg/L苯扎氯铵与500 mg/L茶树精油卫生湿巾的杀菌效果及安全性进行实验室评价。结果将实验指标菌直接污染在卫生湿巾片上作用10 min,对大肠埃希菌、金黄色葡萄球菌和白色念珠菌的杀灭率依次为98. 41%、99. 23%和96. 54%;作用20 min,对大肠埃希菌和金黄色葡萄球菌杀灭率均达到100%,对白色念珠菌杀灭率为99. 32%。用该卫生湿巾擦拭物体表面和人体前臂皮肤,可以清除大肠埃希菌和白色念珠菌对数值均> 3. 0。该卫生湿巾对豚鼠皮肤无刺激性。结论该卫生湿巾具有很好的杀菌效果,对豚鼠皮肤无刺激。

Objective To study the germicidal efficacy and toxicity of a kind of compound benzalkonium chloride wet tissue. Methods Carrier quantitative germicidal test,field simulation test,stability test and skin irritancy test were carried out to observe its germicidal efficacy and toxicity. Results The wet tissue impregnation solution was consisted of 100 mg/L benzalkonium chloride and 500 mg/L tea tree essential oil. All the experimental bacteria were directly contaminated on the wet tissue. The killing rates of Escherichia oli,Staphylococcus aureus,Candida albicans was 98. 41% 、99. 23% 、96. 54% respectively after 10 min,and the killing rates of Escherichia oli,Staphylococcus aureus,Candida albicans was 100% 、100% 、99. 32% respectively after 20 min. The average killing logarithm values of Escherichia coli and Candida albicans was > 3. 00,after wipe test on the contamination table and skin. The rate of skin irritancy test on guinea pigs skin was 0. Conclusion The compound benzalkonium chloride wet tissue has excellent germicidal efficacy,and is non-irritative to skin.