不同清洗剂对消化内镜生物膜的清除效果研究

Study on cleaning effect of biofilm in digestive endoscope by different cleaning agents

目的观察不同清洗剂对消化内镜生物膜的清除效果。方法采用细菌定量检测法和残留蛋白检测法,对不同清洗剂清除模拟内镜管腔人工生物膜的效果进行观察与评价。结果用全效多酶清洗剂、无酶清洗剂和生物膜清洗剂等3组清洗剂清洗后,用细菌培养法检测生物膜清除率依次分别为93. 54%、96. 01%和99. 29%;用残留蛋白检测法检测清除率分别为83. 72%、90. 35%和95. 74%; 3组清除率差异有统计学意义(P <0. 05)。结论本研究3组清洗剂对模拟内镜管腔人工生物膜清除效果,以生物膜专用清洗剂清除效果最好。

Objective To study the cleaning effect for digestive endoscope biofilm by different cleaning agents. Methods The cleaning effect of the biofilm model of endoscope with different cleaning agents were observed and evaluated by means of the bacterial culture and protein quantitative method. Results Using multiple enzymes cleaning agent,no-enzyme cleaning agent and biofilm cleaning agent,the eliminating rates of the bacterial biofilms by means of the bacterial culture were 93. 54%,96. 01% and 99. 29% respectively. The eliminating rates of the bacterial biofilms by means of the protein quantitative were 83. 72% 、90. 35% and 95. 74% respectively,with statistical significant differrence( P < 0. 05). Conclusion The cleaning effect of biofilm cleaning agent was the best among the three cleaning agents on the biofilm model of endoscope.