过氧化氢杀菌试验中泡沫的影响及试验方法的研究

Study on the influence and elimination of foam in hydrogen peroxide germicidal test

目的研究杀菌试验中产生的泡沫对过氧化氢消毒液杀菌效果的影响及其试验方法。方法通过比较载体定量杀菌实验和悬液定量杀菌实验的方法,对实验中产生的泡沫影响过氧化氢消毒液杀菌的效果进行观察。结果不锈钢片载体法定量杀菌试验中,过氧化氢消毒液能与目标细菌充分接触和作用,可以避免泡沫的影响,能准确评价常用浓度过氧化氢消毒液的杀菌性能。而布片、纸片载体可吸附大量气泡,悬液试验同样产生气泡影响试验效果。结论针对某些易被触酶分解产生泡沫的过氧化氢消毒剂,采用不锈钢菌片浸泡定量杀菌试验法评估其杀菌效果。

Objective To study the influence of the foam on the germicidal effects of hydrogen peroxide and the method of elimination. Methods Carrier quantitative test and suspension quantitative test were used to observe the influence of foam on germicidal effect of hydrogen peroxide. Results By carrier quantitative germicidal test with the stainless steel as the carrier,the hydrogen peroxide could fully interact with the target bacteria,avoiding the effects of the foam,so that we could assess the antiseptic performance of hydrogen peroxide accurately. Conclusion For some hydrogen peroxide disinfectants which are easy to be dissociated by thixidase to produce foam,the quantitative germicidal test method of soaking stainless steel bacteria tablets could be used to evaluate its germicidal effect.