两种采样方法对洁净手术室空气细菌监测结果比较分析

Analysis on results of two methods for sampling of bacterial counts of air in clean operating rooms

目的比较沉降法和撞击法在洁净手术室空气细菌监测结果,分析2种采样方法的应用价值。方法采用沉降法和撞击法分别对天津市部分医疗机构洁净手术室室内空气中细菌数监测结果进行比较分析。结果 73间不同级别洁净手术室内空气监测结果表明,平板沉降法监测细菌总数为0～215 cfu/m^3;撞击式采样器法监测细菌总数范围为0～453 cfu/m^3,2组细菌总数间的差异有统计学意义(P <0. 01); 2种监测方法所得数据间存在显著相关性,但2组间空气质量监测合格率差异无统计学意义(P=0. 09)。结论撞击采样器采样法对空气中细菌捕获率高于平板沉降法,但2组采样方法空气质量监测结果无明显差别。

Objective To compare the results of precipitation method and impacting method for sampling of bacterial counts of air in clean operating rooms,and to analyze the practical value of this two different monitoring methods.Methods Bacterial counts of air in part of medical institutions,clean operating rooms in tianjin were sampled by precipitation method and impacting method simultaneously,and the results were comparatively analyzed.Results The results of bacterial counts in the 73 indoor air of clean operating rooms showed that:the range of the bacterial counts of air samples collected by precipitation method were 0~215 cfu/m^3 and by impacting method were 0~453 cfu/m^3,and the bacterial counts had significant difference(P<0.01).the results collected by precipitation method were related to those by impacting method at a certain degrees.however,the qualified rate monitoring by those two methods had no significant difference(P=0.09).Conclusion Compared with the precipitation method,the impacting method has the higher collection efficiency of bacterial counts in monitoring the clean operating room air,but the results monitoring by those two methods have no significant difference.