曲尼司特对兔增生性瘢痕组织的抑制作用及其机制研究

Inhibition Effects of Tranilast on Hypertrophic Scar Tissue of Rabbits and Its Mechanism Study

目的:研究曲尼司特对兔增生性瘢痕组织的抑制作用及其机制。方法:取兔建立增生性瘢痕模型,将建模成功的兔随机分为模型对照组(生理盐水)和曲尼司特低、中、高剂量组(0.3、0.5、0.7 mg/kg),每组6只,局部sc相应药物。各组兔分别于注射前1 h和注射后第1、3、5周测量瘢痕厚度,观察注射后第5周瘢痕病理学变化,并检测瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白表达。结果:与注射前1 h比较,除模型对照组外,其余各组兔注射后各时间点瘢痕厚度均减小(P<0.05)。注射后第5周,与模型对照组比较,其余各组兔注射后第5周的瘢痕厚度均减小,病理学变化均好转,瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白表达均降低(P<0.05),且与曲尼司特剂量呈正相关。结论:曲尼司特能够抑制增生性瘢痕的形成,其作用机制可能与抑制瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白的表达有关。

OBJECTIVE:To study the inhibition effects of tranilast on hypertrophic scar tissue of rabbits and its mechanism.METHODS:Rabbits were selected to induce hypertrophic scar(HS)model,the HS model rats were randomly divided into model control group(normal saline),tranilast low- dose,medium- dose,high- dose groups(0.3,0.5,0.7 mg/kg),6 rabbits in each group,local intradermaly injected corresponding drugs. Scar thickness 1 h before injection and the first,third,fifth week after injection in each group were measured,pathological changes of scar(fifth week after injection)were observed,transforming growth factor β_1(TGF-β_1),α-smooth muscle actin(α-SMA)mRNA and protein expression were detected. RESULTS:Compared with 1h before injection,scar thickness of rabbits in other groups were decreased after injection except for model control group. In fifth week after injection,compared with model control group,scar thickness of rabbits in other groups were decreased,pathological changes were improved;TGF-β_1,α-SMA mRNA and protein expression were decreased(P<0.05),showing positive correlation with tranilast dose. CONCLUSIONS:Tranilast can inhibit the formation of hypertrophic scar,and the mechanism may be related to inhibiting the TGF-β_1,α-SMA mRNA and protein expression.